Fig. 1: Piperazine-based lipids formulate into stable lipid nanoparticles (LNPs).
From: Piperazine-derived lipid nanoparticles deliver mRNA to immune cells in vivo
a Design of ionizable lipids with piperazine backbone. b Procedure to synthesize PPZ lipids. c Composition of the LNP library: 8 different PPZ lipids, 2 cholesterol variants (cholesterol and 20α-OH cholesterol), 2 PEG-lipid variants (C14PEG2K and C18PEG2K), and DOPE. d Each of the compounds was formulated using four molar ratios. e Of the 128 LNPs that were formulated, 65 passed the quality control criteria, with a diameter less than 200 nm as well as a stable autocorrelation curve. f Hydrodynamic diameters of LNPs formulated with PPZ lipids, average ± SEM. g Hydrodynamic diameters of LNPs formulated with cholesterol and 20α-OH cholesterol, average ± SEM. h Hydrodynamic diameters of all administered LNPs; the diameter of the LNP pooled control is within the range of the LNPs composing the pool.
