Fig. 5: Bioreactor profiles from strains QP478 and LC224 in fed-batch mode.

a, b Bacterial growth, glucose and xylose utilization, and muconate titers, yields, and rates from QP478 and LC224 in 96.6-h cultivations. c Bacterial growth, glucose and xylose utilization, and muconate titer, yields, and rate from LC224 in 191-h cultivation. For a and b, data points represent the average values of biological duplicates, error bars represent the absolute difference between the data generated from duplicates at each time point; for (c), data points represent the values of singlets. Metabolic yields (mol%) at each time point were calculated as the amount of muconate (in moles) produced divided by the glucose and xylose (in moles) utilized. Metabolic yields (mol%) are corrected based on the dilution factor generated by the volumes of base and substrate feeding. Final carbon yields (carbon%) listed were calculated as [mM muconate × 6/mM (glucose × 6 + xylose × 5) × 100]. Rates (g L⁻¹ h⁻¹) at each time point were calculated as the muconate concentration divided by the cultivation time. All the titer (T), rate (R), and yield (Y) values listed were at the last time point. Final yields (mol%, carbon%) listed in (c) have also been corrected based on the quantified evaporated volume. Source data are provided as a Source Data file.