Fig. 3: Expression dynamics of auxin-related genes upon SL treatment.
From: Strigolactones are chemoattractants for host tropism in Orobanchaceae parasitic plants
Expression patterns of the DR5 (a–h) and PjPIN2 (i–p) promoters driving expression of a fluorescent marker gene upon treatment with 1 µM rac-strigol (STR) or 0.1% (v/v) DMSO solutions. Filter paper disks were placed 5-mm to the left of the roots. Confocal photos were taken at the indicated time points after treatment on media with (1/2MS + suc) or without (agar) nutrient. Bright-field and fluorescent images were merged in a–h. Venus fluorescence intensity was represented in a Rainbow RGB spectrum in i–p using ImageJ71. White ovals indicate asymmetrical expression. The scale bars in a and i also refer to b–h and j–p, respectively. Bars = 100 µm. At least six biological replicates were performed for each condition with similar results.
