Fig. 5: Long-read capture RNA-sequencing for CDS incomplete isoforms. | Nature Communications

Fig. 5: Long-read capture RNA-sequencing for CDS incomplete isoforms.

From: Splicing QTL analysis focusing on coding sequences reveals mechanisms for disease susceptibility loci

Fig. 5

a Overview of long-read capture RNA-sequencing analysis for CDS incomplete isoforms. b Comparison of long-read capture RNA-sequencing and conventional long-read RNA-sequence. c Results of long-read capture RNA-sequencing for three genes. A compressed light blue horizontal line in the alignment track corresponds to a single read. Gene models are shown for the reference isoform and major unannotated isoform(s) with CDS incomplete isoform specific junction. d Comparison of reference isoforms and completed CDS incomplete isoforms. e Predicted protein structures using ColabFold; BST1-201 and BST1-205, BST1 homodimer (BST1-201) and heterodimer (BST1-201 and BST1-205). f Predicted protein structures using ColabFold; CARD9-201 and CARD9-208, CARD9 homodimer (CARD9-201) and heterodimer (CARD9-201 and CARD9-208). g The ratio of ATXN2L isoforms with alternative TSS to those with reference TSS for each isoform with reference or alternative splice acceptor. h Predicted protein structures using ColabFold; ATXN2L-225 and ATXN2L-221. CDSI CDS incomplete, TSS Transcription start site, SA Splice acceptor.

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