Fig. 2: HURP-gaps are precisely linked to K-fibre growth state.
From: Evidence for a HURP/EB free mixed-nucleotide zone in kinetochore-microtubules
A Lattice light-sheet 3D representation (X-, Y- and Z-projections) of a metaphase hTERT-RPE1-EGFP-HURP/HaloTag-CENP-A cell and relative movie stills projected in Z (90 z-slices). Scale bar = 5 µm. B Lattice light-sheet movie stills of the same cell in A projected in Z (11 z-slices). Scale bar = 5 µm. C Exemplary HURP intensities and sister-kinetochore positions in single pair over time, obtained after 3D kinetochore tracking. D Average spatial distribution of HURP along K-fibre over time relative to switch from leading to trailing (N = 3, n = 69 pairs). E Probability of directional switch in the next frame versus HURP on the K-fibre in the current frame (N = 3, n = 69 pairs). Source data for all graphs are provided as a Source Data file.
