Fig. 1: AEP knockout improves trabecular bone density in ovariectomy female mice. | Nature Communications

Fig. 1: AEP knockout improves trabecular bone density in ovariectomy female mice.

From: A TrkB agonist prodrug prevents bone loss via inhibiting asparagine endopeptidase and increasing osteoprotegerin

Fig. 1

Femoral bone structures were assessed by in vitro μCT in AEP wild-type, AEP knockout (AEP KO) mice with or without ovariectomy. a Representative images of the femoral indices of trabecular bone structure measured by in vitro μCT scan. b μCT scanning measurements of trabecular bone volume fraction (BV/TV), connectivity density (Conn.D), structure model index (SMI), trabecular number (Tb.N), trabecular spacing (Tb.Sp), trabecular thickness (Tb.Th). Data are shown as mean ± SEM, n = 5 mice per group for AEP WT sham group, n = 6 mice per group for AEP KO group and n = 7 mice per group for AEP WT and AEP KO OVX group, one-way ANOVA. c μCT scanning measurements of cortical bone cortical area (Ct.Ar), average cortical thickness (Ct.Th) and relative cortical bone area to tissue area (Ct.Ar/Tt.Ar). Data are shown as mean ± SEM, n = 5 mice per group for AEP WT sham goup, n = 6 mice per group for AEP KO group and n = 7 mice per group for AEP WT and AEP KO OVX group, one-way ANOVA. d Serum levels of osteocalcin (a marker of bone formation), C-terminal telopeptide (CTX) (a marker of bone resorption), RANK-L, OPG, RANK-L/OPG ratio and serum BDNF level in wild-type and AEP knock out mice with and without ovariectomy. Data are shown as mean ± SEM, left to right: n = 5, 5, 7, 7 mice per group for osteocalcein and CTX measurement, n = 5 mice per group for BDNF, RANK-L, and OPG measurement, one-way ANOVA.

Back to article page