Fig. 3: Ice nucleation assay data from PbINP repeat deletions expressed in E. coli.
From: Water-organizing motif continuity is critical for potent ice nucleation protein activity
a, b Fraction of frozen droplets (fice) vs. temperature from the WISDOM and BINARY assays, respectively. The values alongside or above the colored plots refer to the number of central domain repeats remaining in that construct. Each sample’s data points are depicted identically in parts A and B; gold dashed line for 15 repeats, red solid line for 18 repeats, etc. c The relationship between T50 values and the number of INP repeats in the central domain. Data from BINARY and WISDOM are presented in blue-filled circles and red diamonds, respectively; the background freezing temperature of pure LB media/buffer samples for each apparatus is indicated by a horizontal line.
