Fig. 4: Interaction between DPF3a and SNIP1.

a DPF3a interacted with SNIP1. Immunoprecipitation was carried out using antibody against HA or SNIP1, followed by immunoblotting (IB) to detect endogenous SNIP1 using anti-SNIP1 antibody or HA-tagged DPF3a/b using the anti-HA antibody in 786-O cells expressing empty vector, HA-tagged DPF3a or HA-tagged DPF3b. Input lanes represents 5% of total protein lysate. b Co-localization of DPF3a and SNIP1 in the nucleus. 786-O cells expressing HA-tagged DPF3a were fixed with methanol and stained with antibodies against HA (red) and SNIP1 (green). Scale bar, 10 μm. c DPF3a interacted with the N-terminal domain of SNIP1. HA-tagged DPF3a were co-transfected separately with Flag-tagged full-length SNIP1 or its C-/N- terminus in HEK293T cells. HA-tagged DPF3a was purified by immunoprecipitation with anti-HA magnetic beads, followed by immunoblotting to detect Flag-tagged SNIP1 truncations with the anti-Flag antibody. d SNIP1 interacted with the C-terminus of DPF3a. Flag-tagged SNIP1 were co-transfected separately with full-length HA-tagged DPF3a or its C-/N- terminus in HEK293T cells. Flag-tagged SNIP1 was purified by immunoprecipitation with anti-Flag magnetic beads, followed by immunoblotting to detect HA-tagged DPF3a with the anti-HA antibody. a–d Three independent experiments were performed and similar results were obtained. Source data are provided in the Source Data file.