Fig. 4: HB maintains erythropoiesis at three distinct developmental stages. | Nature Communications

Fig. 4: HB maintains erythropoiesis at three distinct developmental stages.

From: Single-cell analysis of hepatoblastoma identifies tumor signatures that predict chemotherapy susceptibility using patient-specific tumor spheroids

Fig. 4

a Integrated UMAP of fetal liver erythroid cells and tumor-associated erythroid cells. b Heatmap of the three developmental stages of fetal-liver erythroid marker expression for tumor-associated erythroid cells and reference fetal liver erythroid population. Scaled expressed levels are shown by the color bar. c Supervised heatmap showing expression of canonical erythroid markers and representative markers of three erythroid developmental stages. d Selected ligand-receptor interactions from TAMs to tumor-associated erythroid cells for patients 2, 5, 6, and other patients. Mean expressions of ligand and receptor pairs are shown in the color bar. Statistical significance levels (random permutation test) are indicated by the marker size. e Stainings for TAMs to tumor-associated erythroid cells ligand-receptor interactions by FISH with CD163 (red), HBA2 (blue), VCAM1 (white), and ITGA4 (green) on patient 6 tumor tissue, and violin plot of VCAM1 and ITGA4 for TAMs and tumor-associated erythroids from patient 6 tumor tissue. Arrows show TAMs expressing VCAM1 next to tumor-associated erythroids expressing ITGA4. f Selected ligand-receptor interactions between tumor cells and tumor-associated erythroid cells for patients 2, 5, 6, and other patients. Mean expression of ligand and receptor pairs are shown in the color bar. Statistical significance levels (random permutation test) are indicated by the marker size. g Stainings for tumor cells to tumor-associated erythroid cells ligand−receptor interactions by FISH with MEG3 (red), HBA2 (blue), IGF2 (white), and IGFR1 (green) on patient 6 tumor tissue, and violin plot of IGF2 and IGFR1 for tumor Hepatoblast I and tumor-associated erythroids from patient 6 tumor tissue. Arrows show tumor cells expressing IGF2 next to tumor-associated erythroids expressing IGFR1. h Stainings for tumor cells to tumor-associated erythroid cells ligand−receptor interactions by FISH with REG3A (red), HBA2 (blue), SPP1 (white), and ITGA4 (green) of patient 2 tumor tissue, and violin plot of SPP1 and ITGA4 for tumor Erythroid-like and tumor-associated erythroids from patient 2 tumor tissue. Arrows show tumor cells expressing SPP1 next to tumor-associated erythroids expressing ITGA4. Scale bar = 35 µm.

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