Fig. 6: E-cadherin in DTC awakening. | Nature Communications

Fig. 6: E-cadherin in DTC awakening.

From: Epithelial-mesenchymal plasticity determines estrogen receptor positive breast cancer dormancy and epithelial reconversion drives recurrence

Fig. 6

a Day 1 radiance of MCF-7 control and CDH1 cells. Data represent mean ± SD, n = 16, 17 glands in 4, 5 mice. Unpaired Student’s t-test. b Radiance fold-change. Data represent mean ± SEM, n = 16, 17 glands in 4, 5 mice c Relative CDH1 mRNA levels in MCF-7 control and CDH1 cells, n = 5. Data represent mean ± SD. Unpaired Student’s t-test. d Ex vivo lung radiance. Data represent mean ± SD from 5 control and 4 CDH1 mice. Multiple t-tests. e Fluorescence micrographs of MCF-7 control (top) or CDH1 (bottom) lungs. Scale bar, 500 µm. f Experimental scheme. g, h Relative radiance and weight (g) of 20 xenografted glands from -DOX and +DOX mice. Data represent mean ± SD. Unpaired Student’s t-test. i Fold-change lung radiance in - or +DOX, Data represent mean ± SEM, n = 16 in each group. Two-tailed Mann-Whitney test. **p ≤ 0.01. j Relative micro-metastatic burden. Data represent mean ± SEM, n = 69 from 16 mice/group. Nonparametric Mann-Whitney Test. k Representative fluorescence micrographs of lungs from - and +DOX mice. Scale bars, 500 µm. l Quantification of lung lesion area from - or +DOX mice, n = 9 each. n≥476 lesions 9 mice/condition. Data represent mean ± SD. Unpaired Student’s t-test. m Representative micrographs of lung sections from - or +DOX mice. Scale bar, 100 µm. n Quantification of lesion length and area in H&E stained lung sections from - and +DOX, n ≥ 30 lesions, 3 mice/condition. Data represent mean ± SD. Unpaired Student’s t-test. o. Relative E-cad intensity (Int.) and representative IF of E-cad in lung lesions, n ≥ 38 from 3 mice/condition. Scale bar, 50 µm. Data represent mean ± SEM. Unpaired Student’s t-test. p Relative CDH1 and MKI67 mRNA levels in lungs from 6 -DOX and 7 +DOX mice. Data represent mean ± SD. Nonparametric Mann Whitney test. q Pearson plot showing correlation between MKI67 and CDH1 mRNA levels in 6 -DOX and 7 +DOX mice. r Relative mRNA levels of EMT-TFs in lungs from 5 -DOX and 6 +DOX mice. Data represent mean ± SD. Nonparametric Mann Whitney test. All relative mRNA levels were normalized to GAPDH.

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