Fig. 1: Nanopore detection of DNA circuit outputs.

a DSD circuit detection with a nanopore sensor. Circuit components are mixed and loaded onto a nanopore sensor array for real-time readout. Input strands react with the gate complex displacing the barcoded and 3’ labeled biotin-streptavidin output ssDNA, which is then free to be captured and read by a nanopore sensor. The nanopore sensor array is capable of distinguishing different output strand barcodes, enabling circuit multiplexing. b Example raw nanopore data showing repeated capture and ejection of biotinylated ssDNA output strands. DNA capture events manifest ionic current drops from open-pore to a lower ionic current level. Strands are ejected from the pore by reversal of the applied voltage. c Histogram showing the distribution of mean fractional current from capture events belonging to three samples: nanopore running buffer, running buffer with 4 uM streptavidin, and running buffer with both 4 uM streptavidin and 0.5 uM 3’ biotinylated ssDNA. d Standard curve showing the relationship between average time between output strand capture and strand concentration. Error bars represent ± standard deviation of three replicates, with each replicate using a different barcoded strand.