Fig. 3: Comparison of the RhoGAP binding sites for RhoA and p120RasGAP SH3 domain.

a Surface of DLC1 RhoGAP domain with p120RasGAP SH3 domain binding site highlighted in blue. The Arginine finger Arg-1114 is shown in blue and labeled, and two surface residues mutated in this study (Thr-1223 and Leu-1267) are shown in red. b Surface of p190RhoGAP with RhoA binding site highlighted in teal (PDB ID: 5IRC⁷). The Arginine finger Arg-1284 is shown in red and labeled. Views in A and B are similar after superposition of the RhoGAP domains. Interface residues in (A) and (B) are defined by CCP4mg⁸⁴ and is based on buried surface. c Structure of RhoA in complex with p190RhoGAP (PDB ID: 5IRC, gray and yellow) superposed with p120RasGAP SH3 in complex with DLC1 (green and purple). Superposition on the RhoGAP domains. The p120RasGAP SH3 domain (purple) is shown as ribbon and transparent surface to highlight the overlap in binding sites. Inset: the SH3 domain RT loop is a close mimic of the switch I and switch II regions of the active (GTP-bound) conformation of RhoA. The location of the arginine finger Arg-1114 Ca is indicated as a sphere.