Fig. 1: Red1 forms multiple interactions within the MTREC complex.
From: Structural analysis of Red1 as a conserved scaffold of the RNA-targeting MTREC/PAXT complex
a Schematic representation of the MTREC complex. Red1 and Mtl1 form the MTREC complex core7. b Y2H assays revealed that Red1 interacts with Ars2 and Iss10. Mtl1 was used a positive control. Red1 was used as bait. Growth assayed on selective medium lacking histidine and with 50 mM 3-amino-1,2,4-triazole (3AT) histidine inhibitor is shown. EV- empty vector, BD- binding domain, AD- activating domain. c Y2H analysis of Red1 self-association. Growth was assayed on medium lacking histidine and with 30 mM 3AT. d Schematic representation of the interactions characterized in this study.
