Fig. 8: MTREC/PAXT complex model.
From: Structural analysis of Red1 as a conserved scaffold of the RNA-targeting MTREC/PAXT complex
a, b Schematic models of the MTREC and PAXT complexes, summarizing the structural and biochemical information obtained in this study and in ref. 14. The crystal structure between Mtr4 and Red1 was determined using C. thermophilum proteins and the key interacting residues are conserved in S. pombe Red1 and Mtl1 as well as in human MTR4 and ZFC3H114. AlphaFold235 structure prediction of the human complex structure (Supplementary Fig. 15) indicates that that MTR4-binding region of ZFC3H1 might pack against a small helical domain. It is thus likely, that the MTR4-binding domain of ZFC3H1 covers residues 1140–1292. Similarly, in the AlphaFold2 prediction of Red1 (AF-Q9UTR8), this domain spans residues 575–703.
