Fig. 1: Mycobacterial lipids that can significantly increase LysoTracker™ staining of RAW 264.7 MΦ are widely distributed across Mtb clinical strains, members of the Mtb complex (MTBC) and non-tuberculous mycobacteria. | Nature Communications

Fig. 1: Mycobacterial lipids that can significantly increase LysoTracker™ staining of RAW 264.7 MΦ are widely distributed across Mtb clinical strains, members of the Mtb complex (MTBC) and non-tuberculous mycobacteria.

From: Inhibition of the Niemann-Pick C1 protein is a conserved feature of multiple strains of pathogenic mycobacteria

Fig. 1

a LysoTracker™ staining intensity of RAW 264.7 MΦ treated with 100 μg/ml lipid extract of clinical Mtb strains representative of Mtb lineages, 1, 2, and 4, Mtb complex species and non-tuberculous mycobacteria for 48 h. Data are mean ± SEM, N = minimum of 2 replicates per sample. Statistical analysis, 1-way ANOVA, ****p <0.0001, other significance values as indicated. Data are representative of three independent experiments. b Mean LysoTracker™ staining intensity of RAW 264.7 MΦ treated with lipid from Mtb lineages, 1, 2, and 4. Data are mean ± SEM, N = 5–7 strains tested per lineage. Statistical analysis, 1-way ANOVA, ****p < 0.0001, other p values as indicated. Data are representative of three independent experiments. c LysoTracker™ staining of RAW 264.7 MΦ treated with 200 μg/ml lipid extract of clinical Mtb strains from lineages 1, 2, 3, and 4 and M. canettii. Data are mean ± SEM, N = minimum of 3 replicates per sample. Statistical analysis, 1-way ANOVA, ****p < 0.0001, other p values as indicated. Data are representative of two independent experiments. Source data are provided as a Source data file.

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