Fig. 6: Mtb lipids affect GM1 trafficking in RAW 264.7 MΦ. | Nature Communications

Fig. 6: Mtb lipids affect GM1 trafficking in RAW 264.7 MΦ.

From: Inhibition of the Niemann-Pick C1 protein is a conserved feature of multiple strains of pathogenic mycobacteria

Fig. 6

a Confocal microscopy images of RAW 264.7 MΦ treated with vehicle (DMSO), U18666A, or mycobacterial lipids and pulse-chased with cholera toxin B subunit (green). Nucleus is stained with DAPI (blue). For each pair, right image is a higher magnification of the region outlined in the left image. Untreated, vehicle and M. canettii lipid treated cells show a predominantly Golgi pattern of staining, indicated by the white arrows. U18666A and lipids from Mtb strains induce a punctate pattern, consistent with lysosomal distribution (white arrowheads). Scale bar represents 10 μm. b Percentage of vehicle and U18666A treated cells with lysosomal cholera toxin B localization. Data are mean ± SEM, N = 3, each representing a minimum of 100 cells in randomly selected fields. Statistical analysis, unpaired t test with Welch’s correction, two-tailed. ****p < 0.0001. c Percentage of the vehicle and mycobacterial lipid-treated cells with lysosomal cholera toxin B localization. Data are mean ± SEM, N = 3, each representing a minimum of 100 cells in randomly selected fields per sample. Statistical analysis, unpaired t test with Welch’s correction, two-tailed. ****p < 0.0001, other p values as indicated. Data are representative of two independent experiments. Source data are provided as a Source data file.

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