Table 2 Summary of relative accumulation of total and specific GSL species in RAW 264.7 MΦ treated with U18666A or lipid extracts from different Mtb clinical strains and M. canettii

Lac

****

6.72 ± 0.35

*0.0151

1.64 ± 0.16

ns

1.05 ± 0.03

ns

1.17 ± 0.17

*0.0179

1.90 ± 0.24

**0.0054

0.60 ± 0.08

GA2

****

7.46 ± 0.37

**0.0069

1.62 ± 0.10

ns

0.93 ± 0.07

ns

1.17 ± 0.31

ns

1.23 ± 0.12

ns

0.88 ± 0.08

Gb3

***0.004

3.19 ± 0.27

ns

1.62 ± 0.16

ns

1.42 ± 0.12

ns

1.51 ± 0.28

*0.0054

2.40 ± 0.07

ns

1.01 ± 0.12

GM3

****

8.77 ± 0.63

****

1.96 ± 0.05

*0.0111

1.40 ± 0.10

ns

1.52 ± 0.10

**0.0022

1.83 ± 0.13

***0.002

0.62 ± 0.02

GM2

****

41.35 ± 2.82

****

2.82 ± 0.08

***0.0007

2.02 ± 0.11

***0.008

2.08 ± 0.10

****

5.21 ± 0.12

**0.0084

1.20 ± 0.04

GA1

****

4.96 ± 0.33

****

2.24 ± 0.07

****

1.88 ± 0.06

****

1.82 ± 0.06

****

2.23 ± 0.05

*0.0117

1.23 ± 0.04

GM1a

***0.0006

2.13 ± 0.15

****

2.02 ± 0.08

***0.0009

1.81 ± 0.11

****

1.75 ± 0.06

****

1.56 ± 0.03

ns

1.02 ± 0.03

GM1b

**0.0071

1.81 ± 0.14

***0.0002

1.55 ± 0.06

**0.0067

1.41 ± 0.09

***0.0007

1.30 ± 0.03

***0.0005

1.31 ± 0.03

**0.0043

0.79 ± 0.02

GD1a

ns

1.27 ± 0.10

*0.0382

1.18 ± 0.05

ns

1.17 ± 0.08

ns

1.06 ± 0.04

ns

0.95 ± 0.03

**0.0016

0.62 ± 0.02

Total GSL

***0.0003

2.44 ± 0.17

***0.0002

1.56 ± 0.06

**0.0042

1.44 ± 0.09

***0.0008

1.34 ± 0.05

**0.0011

1.28 ± 0.03

**0.0048

0.79 ± 0.03