Fig. 4: KLF4 and BRG1 co-occupy regions with increased accessibility under LSS, most of which are distal enhancers.

a Pie chart depicting the intersection of regions with increased accessibility under LSS by ATAC-Seq, with KLF4 and BRG1 ChIP-Seq data from PAEC transduced with caMEK5. Percentages indicate the fraction of DAR with increased accessibility under LSS vs ST that are differentially enriched for KLF4 and/or BRG1. b Scatterplot showing the correlation between KLF4 binding and accessibility changes at LSS vs ST DAR co-occupied by KLF4-BRG1. Indicated values were calculated by a two-tailed Pearson R test, with P < 0.0001. c ATAC-Seq and KLF4 and BRG1 ChIP-Seq tracks showing enrichment for both factors at the DAR 39 Kb upstream of DUSP5. d PAEC were treated with siRNA targeting BRG1 (siBRG1) or with non-targeting controls (siC) prior to exposure to 15 dyn/cm² of LSS or ST conditions for 24 h. Bar graphs indicate accessibility of the DAR 39 Kb upstream of DUSP5, and DUSP5 gene expression, both assessed by qPCR. e Venn diagram showing the percentage of KLF4-BRG1 co-occupied DAR with increased accessibility under LSS, that are enriched for H3K4me1 and H3K27ac. n = 2 experimental replicates for a–c, e, and n = 4 experimental replicates for d. Data are shown as the mean ± s.e.m. P values were determined by Student’s two-tailed t-test. Source data are provided as a Source Data file.