Fig. 7: The MCM9-BRCv motif is influential in stabilizing HU-stalled forks, but the ATPase activity is necessary for normal fork progression.
a Schematic of MCM9 showing domains and individual motifs. b 9KO cells (○) were transfected pEGFPC2-MCM9(FR687/8AA) (BRCv−) (
, brown) or pEGFPC2-MCM9(K358A) (WA−) (
, pink) for 48 h prior. Cells were then sequentially incubated with CldU and IdU for the indicated time intervals or followed by 2 mM HU for 4 h (● or ■). DNA was spread by gravity to measure NSD by quantifying IdU and CldU fiber lengths (>150 fibers) using Image J, and the corresponding median ratios are reported at the top of the plots and by a black line embedded in the data. Representative fibers are shown to the right of the dot plots. A Mann–Whitney two-sided U test was used to calculate P values (n.s. nonsignificant, **P < 0.01, ***P < 0.001, ****P < 0.0001).
