Table 2 Comparison of tracer 1 and tracer 7

From: Rational design of a sensitivity-enhanced tracer for discovering efficient APC–Asef inhibitors

Tracer

EC50 [µM]

Kd [µM]

Protein conc. [µM]

Tracer conc. [nM]

Span [mp]

Z’

1

0.54 ± 0.009

0.58

1.0

20

134

0.83

7

0.016 ± 0.0003

0.078

0.025

20

251

0.92

  1. The experiments were performed in triplicates and repeated twice (n = 2 independent experiments). Source data are provided as a Source Data file.
Back to article page