Fig. 5: Single-cell landscape of CD8+ T cells in Lamina Propria of human small intestine from Ctrl, CeD and GFD samples. | Nature Communications

Fig. 5: Single-cell landscape of CD8+ T cells in Lamina Propria of human small intestine from Ctrl, CeD and GFD samples.

From: Single cell transcriptomic analysis of the immune cell compartment in the human small intestine and in Celiac disease

Fig. 5

a UMAP clustering depicting the heterogeneity of CD8+ T cells in Lamina Propria (LP) from Ctrl (control subjects), CeD (Celiac disease patients) and GFD (Celiac patients on gluten-free diet) samples. b Projection of cells according to Ctrl, CeD and GFD origin on the UMAP clusters (left scatter plot) and the boxplots showing the distribution of cells across the clusters per each condition as compared to each other (right) (center line, median; box limits, upper and lower quartiles; whiskers, minimum and maximum values; points, outliers, 1.5x interquartile range; two-sided t-test p value < = 0.05 (C0: Ctrl vs CeD = 3.45 × 10−8, CeD vs GFD = 1.30 × 10−8; C1: Ctrl vs CeD = 7.15 × 10−7, CeD vs GFD = 3.14 × 10−9; C2: Ctrl vs GFD = 4.56 × 10−5, CeD vs GFD = 0.0004; C3: Ctrl vs GFD = 1.51 × 10−7, CeD vs GFD = 7.38 × 10−8); n = 10 randomized cell subsampling). c Heatmap plot of the mean expression of top differentially expressed genes per each CD8+ T-cell cluster (empirical Bayes two-sided p value < = 0.05). d Heatmaps depicting the gene expression of the cytokine ligands (left) and receptors (right) between the clusters of CD8+ T cells (differentially expressed genes per each cluster are shown in bold).

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