Fig. 2: FTY720 treatment in the first weeks of ART retains cytolytic cells but does not enhance their functionality in the LN. | Nature Communications

Fig. 2: FTY720 treatment in the first weeks of ART retains cytolytic cells but does not enhance their functionality in the LN.

From: Limited impact of fingolimod treatment during the initial weeks of ART in SIV-infected rhesus macaques

Fig. 2: FTY720 treatment in the first weeks of ART retains cytolytic cells but does not enhance their functionality in the LN.The alternative text for this image may have been generated using AI.

a Frequency of CD4+ T cells, b CD8+ T cells, and (c) ratio of CD4+/CD8+ T cells in lymph node (LN) mononuclear cells at baseline (week −1) and at 8 weeks of treatment in early FTY720 (n = 8 RMs) or control (n = 14 RMs) SIV-infected RMs. d Frequency of LN perforin+  (Perf+), e granzyme B (GrB+), and (f) Perf+GrB+ CD8+ T cells at baseline (week −1) and at 8 weeks in early FTY720 (red) or control (black) SIV-infected RMs. g Fold reduction between week −1 and week 8 of Perf+GrB+ CD8+ T cells, and (h) frequency of LN CD8+ T cells expressing CXCR5 at baseline (week −1) and at 8 weeks in early FTY720 (n = 8 RMs) and control (n = 14 RMs) SIV-infected RMs. i Representative flow cytometry staining of P185 mastocytoma target cells (TFL4+ cells) expressing active caspase-3 when cultured alone or co-cultured with different ratios of effector CD8+ T cells derived from LN. j Frequency of target cells expressing active caspase 3 following co-culture with CD8+ T cells derived from LN at week 8 from early FTY720 (n = 8 RMs) or control (n = 7 RMs) SIV-infected RMs. k Representative flow cytometry staining of CD56−CD16+NK+ cells in LN at week −1 and 8 from early FTY720 (top) or control SIV-infected RMs (bottom). l Frequency of CD56−CD16+ NK cells and (m) CD16 geometric mean fluorescence intensity (geomean) in NK cells derived from LN at week −1 and 8 from early FTY720 (n = 8 RMs) or control (n = 14 RMs) SIV-infected RMs. ART antiretroviral treatment. Data are presented as the mean ± SD. Statistical differences between FTY720 treated and control groups are indicated with P values and were assessed with a two-sided (95% CI) Mann–Whitney U-test.

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