Fig. 1: Autophagy-deficient CD4+ T cells show delayed proliferation. | Nature Communications

Fig. 1: Autophagy-deficient CD4+ T cells show delayed proliferation.

From: Mapping autophagosome contents identifies interleukin-7 receptor-α as a key cargo modulating CD4+ T cell proliferation

Fig. 1: Autophagy-deficient CD4+ T cells show delayed proliferation.

a Experimental set-up for the generation of bone marrow (BM) chimaeras. Lethally irradiated CD45.1+ hosts were reconstituted with a 1:1 mix of BM of either T-Atg7−/− or wild-type (donors both CD45.2+) and CD45.1+ wild-type BM. After 7 weeks, hosts were intravenously (i.v.) infected with 1 × 106 PFU murine cytomegalovirus (MCMV). Cells from spleens were collected and analysed 7 days post-infection. b Dot plots (left) of splenic MCMV-specific CD4+ T cells within CD19- population from CD45.2+ wild-type or T-Atg7−/− donors and CD45.1+ donors. Bar graph (right) indicates percentage of tetramer (Tet)+ CD4+ T cells within the CD45.2+ CD19- or CD45.1+ CD19- population (n = 8 mice for wild-type chimaera group, n = 9 mice for T-Atg7−/− chimaera group), represented as mean ± SEM with paired two-tailed Student’s t test. c Experimental set-up for the adoptive transfer: OT-II lymphocytes (2 × 104) from either CD45.2 wild-type or T-Atg7−/− mice were adoptively transferred into CD45.1 recipient mice. The recipient mice were orally immunised with 1 × 106 IU recombinant H5 adenovirus expressing ovalbumin (AdH5-OVA). The number of OT-II cells was determined by flow cytometry 8 days after immunisation. d Dot plots (left) are gated on CD45.2+, TCR Vα2+ in the spleens of recipient mice. Bar graph (right) depicts the frequency of gated population within total CD4+ T cell population (n = 5 mice per group). e Splenic OT-II+ T cells from wild-type or T-Atg7−/− mice were stimulated with ovalbumin in culture medium supplemented with murine IL-2 for 1, 3, 7, or 10 days. Histograms by flow cytometry (left) represent OT-II+ CD4+ T cell proliferation. Bar graph (right) indicates the percentage of CellTrace Violet-negative cells (most divided, defined by gate on day 7) within total OT-II+CD4+ T cells (n = 4 mice per group). All data are representative of three independent experiments. d, e are represented as mean ± SEM with unpaired two-tailed Student’s t test. Exact p values are depicted in the figure. Source data are provided as a Source Data file.

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