Fig. 4: Assessment of the effect of ISG65 and C3 knock-out on trypanosome growth in mice.

a The effect of ISG65 knock-out on trypanosome infections in Balb/c mice. Five mice were infected with bioluminescent ISG65+/+ (blue, n = 5 animals) or ISG65−/− (orange, n = 5 animals) T. brucei cell lines. Two uninfected mice were used as controls for basal bioluminescence (grey, n = 2 animals). Trypanosome burden was measured by imaging bioluminescence over time. b The effect of C3 knock-out on trypanosome infections in C57BL/6 mice. Bioluminescent ISG65+/+ T. brucei were used to infect five C57BL/6 mice (blue, n = 5 animals) and five C57BL/6 mice lacking C3 (purple, n = 5 animals). Two uninfected mice were used as controls for basal bioluminescence (grey, n = 2 animals). Trypanosome burden was measured by imaging bioluminescence over time. c The effect of C3 knock-out on ISG65−/− trypanosome infections in C57BL/6 mice. Bioluminescent ISG65−/− T. brucei were used to infect five C57BL/6 mice (orange, n = 5 animals) and five C57BL/6 mice lacking C3 (red, n = 5 animals). Two uninfected mice were used as controls for basal bioluminescence (grey, n = 2 animals). Trypanosome burden was measured by imaging bioluminescence over time. Individual mice are seen in Supplementary Fig. 7. The data presented in a and b were conducted concurrently, while that presented in c was conducted subsequently and should not be directly compared with the data in a and b. Points represent the mean and error bars the standard deviation.