Fig. 3: Adipocyte-specific Atp6v0a1 depletion in vitro and in vivo.
From: Adipocytes control food intake and weight regain via Vacuolar-type H+ ATPase
Atp6v0a1 mRNA quantification in WAT and BAT (f = 7.221, df = 3, p = 0.0013) adipose tissue in KO (white bars) and WT controls (red bars), n = 7 per group. b Western blots and densinometric quantification of Atp6V0a1 in adipocytes (t = 16.86, df = 4, CI = −0.244 to −0.175, p < 0.001) and SVCs (t = 0.41, df = 6, CI = −0.028 to 0.02, p = 0.34) from gonadal WAT from WT (adiponectin Cre) and KO mice (n = 3–4/group). c Food intake (F = 3.03, df = 28, p = 0.03), cre, n = 7, FF n = 12 KO, n = 10. d Body weight (F = 9.86, df = 24, p < 0.001), of adiponectin Cre (Cre, n = 7) (red bars), Atp6v0a1fl/fl (F/F, n = 9) (gray bars) and KO (n = 9, white bars) mice, e weight of WAT (t = 2.416, df = 30, p = 0.02) and liver (t = 0.88 df = 30, p = 0.37) as % of total body weight of WT and KO mice (n = 7–12/group). All mice were fed normal chow. a, c–e were analyzed by one-way ANOVA followed by two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli with false discovery rate of 0.10. b was analyzed by students t-test (2 tailed). Data is expressed as mean ± SEM, *denotes statistical significance at p < 0.05.
