Fig. 9: A salt bridge between Asp945 and Arg949 of ngHKA. | Nature Communications

Fig. 9: A salt bridge between Asp945 and Arg949 of ngHKA.

From: Structure and function of H+/K+ pump mutants reveal Na+/K+ pump mechanisms

Fig. 9: A salt bridge between Asp945 and Arg949 of ngHKA.The alt text for this image may have been generated using AI.

Close-up views of the TM5 and TM8 region where the Na+-binding-site-III-coordinating Asp945 is located in SPWC-ngHKA. The equivalent positions for gHKA and NKA are shown. Asp945 forms a salt bridge (dotted lines) with Arg949 in the E2-Pi structures of WT-, K794A-, and K794S-ngHKA. This salt bridge is also observed in gHKA structures, while obviously absent in SPWC-ngHKA and NKA, due to the replacement of Arg949 with Cys. Comparison of 3Na+·E1-ATP and (2K+)E2-Pi state of SPWC-ngHKA and its equivalent states for NKA (bottom panels) show little displacement in this site-III region during the transport cycle.

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