Table 1 Multiple turnover kinetics, binding affinity, and dissociation kinetics of ptRNA_AU and ptRNA_GG

From: Structural and mechanistic basis for recognition of alternative tRNA precursor substrates by bacterial ribonuclease P

ptRNA

kcat

(s−1)

kcat/Km

(M−1 s−1 × 106)

Kd

(nM) (3 mM Ca2+)

AU

0.12 ± 0.01

7 ± 2

35 ± 5

AU_T5

0.0250 ± 0.0003

1.3 ± 0.1

Nd

AU_D3

0.261 ± 0.009

26 ± 8

Nd

AU_T5D3

0.060 ± 0.003

3.1 ± 0.9

Nd

GG

0.07 ± 0.01

0.5 ± 0.2

63 ± 8

GG_D3

0.048 ± 0.001

4.2 ± 0.9

Nd

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