Fig. 3: Metabolic heterogeneity associates with phenotypic polarization of CD1chi and CD86hi DC populations.
From: Distinct metabolic states guide maturation of inflammatory and tolerogenic dendritic cells
A Correlation analysis for single-cell glycolytic glycolysis and OXHOS scores with heatmap expression single-cell overly of indicated immune markers. Subsampled single-cell data points for the individual donor (out of N = 3) are shown for the entire figure. B Mass cytometry scatter plots for CD1c and CD86 expression profiles were used to emphasize the distribution of CD1chi and CD86hi populations. C Shown are single-cell scatter plot comparisons of the top 4th quantiles from CD1chi (blue) and CD86hi (gold) DC populations. Lower graphs represent histogram distributions of single-cell scMEP metabolic pathway scores in CD1chi and CD86hi populations. D Box plots represent median expression values of glycolytic enzymes and PDK1 in the 1st (lowest, black) and 4th (highest, red) quantile from CD1c and CD86 populations across iDC, actDC, and mDC from 3 independent donors. Statistical significance was calculated using two-sided Student’s t-test. A role for PDK1 in pyruvate to Acetyl-CoA conversion is depicted underneath the graphs. E tSNE visualization of SCENITH profiling depicts clustering of DC stages with CD1c expression heatmap overlay. Adjacent gating strategy was used to select CD1chi and CD86hi populations, whose spatial distribution is emphasized (with matching colors) on tSNE maps divided into separate iDC, actDC, and mDC stages. F Heatmap of gMFI expression for collection of SCENITH phenotyping markers in CD1chi and CD86hi populations from iDC, actDC, and mDC (N = 3 donors). Donor label, DC differentiation stages population frequency, protein synthesis levels along with SCENITH percentual metabolic profiles are annotated. For all panels, P-values are represented as *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. p-values < 0.05 were considered statistically significant (ns). Box plots indicate second and third quantile (box), median (horizontal line) and 1.5× the interquartile range (whiskers). Source data are provided as a Source Data file.
