Fig. 4: Modes of age-associated reduced gene expression in CD8+ T cell subpopulations.
From: Heterogeneity and transcriptome changes of human CD8+ T cells across nine decades of life
a Scatter plots showing examples of gene expression that decrease across aging in each of three modes (EEF2 – percentage, KLRB1 – expression, NUCB2 – both). Trend lines were from the lineage regression model for which the t distribution with n-2 degrees of freedom was used to evaluate if a slope was significant for a particular variable (2 sided t-test). b Scatter plots categorizing genes decreasing across age into three modes of change in nine clusters identified by scRNA-seq (Full gene list is in Supplementary Data 4). c. Bubble plot showing functional gene sets enriched for each of three modes of change across age. Representative genes for each gene set shared across clusters shown. NES, normalized enrichment score. The scale of the magnitude of significance is 0 to 10−5. GO, gene ontology.
