Fig. 8: TET2 overexpression potentiates osteoblastic differentiation.
From: Vitamin C epigenetically controls osteogenesis and bone mineralization
a TET2 western blot after CRISPR-dCas9-mediated TET2 o/e (overexpression) and b 5hmC dot blot under the same conditions. c ECM mineralization after TET2 o/e at day 21 of differentiation. d Osteoblastic gene expression during TET2 o/e. Bar graphs represent mean ± SD; *p < 0.05; **p < 0.01; ***p < 0.001. Unpaired two-tailed t test in (c); two-way ANOVA analysis with Tukey’s multiple comparison tests, groups compared to D0 Ctrl or as marked in d. N = 3 (c, d) per group, from biologically independent experiments. Source data as well as exact p = values for all comparisons in d are provided in the Source Data File.
