Table 1 Oligonucleotide primers used in this study

From: Streptococcus agalactiae cadD alleviates metal stress and promotes intracellular survival in macrophages and ascending infection during pregnancy

Primer name

Sequence (5′ to 3′)

cadD-P1a

CCGCGGATCCCAGTACCTGCACGTCACACT

cadD-P2b

CCCATCCACTAAACTTAACAAAGCCCTGCCATCGAAATGA

cadD-P3b

TGTTTAAGTTTAGTGGATGGGTGCTATGGGCTGTGTTAGGC

cadD-P4c

GGGGGTACCCCTGCATTGCCTAGTTCGCT

cadD-P5

CAGTACCTGCACGTCACACT

cadD-P6

TACGTATAGCTCGAAGCGGT

cadD-pLZ12-F2a

CGCGGATCCAGGAGGACAGCTATGATTCAAAATGTTGTTAC

cadD-pLZ12-R2d

AAAACTGCAGCTAGCCTAACACAGCCCATA

RT-gyrA-F

CGGGACACGTACAGGCTACT

RT-gyrA-R

CGATACGAGAAGCTCCCACA

RT-cadD-F

GCACAAGTCCCTTCTGTTGG

RT-cadD-R

GCCTAACACAGCCCATAGCA

  1. aBamHI restriction site is shown in bold.
  2. bComplementary sequences for P2 and P3 sets are underlined.
  3. cKpnI restriction site is shown in bold.
  4. dPstI restriction site is shown in bold.
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