Fig. 1: In vivo therapeutic efficacy of 12H5 in mice and in vitro neutralization and binding activity of C12H5. | Nature Communications

Fig. 1: In vivo therapeutic efficacy of 12H5 in mice and in vitro neutralization and binding activity of C12H5.

From: Identification of a cross-neutralizing antibody that targets the receptor binding site of H1N1 and H5N1 influenza viruses

Fig. 1: In vivo therapeutic efficacy of 12H5 in mice and in vitro neutralization and binding activity of C12H5.The alternative text for this image may have been generated using AI.

a–d Therapeutic efficacy of 12H5 against lethal challenge of MA-CA7/2009 (a, b), and MA-QH/2005 (c, d). The body weight (a, c) and survival curves (b, d) of BALB/c mice (n = 5 per group) treated with 12H5 or PBS 24 h after lethal challenge are shown. The body weight curves reflect the weights of surviving mice; data pertaining to the mice that died after challenge were omitted from the body weight data. Data in a, c are presented as mean values ± SD. For survival data, the log-rank test was used to evaluate significance, compared to control PBS-treated group (*P < 0.05, ****P < 0.0001). e Binding reactivity of C12H5 to purified HA proteins from H1N1 and H5N1 strains. EC50 values above 104 ng/mL were recorded as unfavorable. Full strain designations are detailed in Supplementary Table 2. f Binding affinity of C12H5 to HAs of three strains. The kinetic constants represent a representative experiment from two independent experiments. Data are presented as mean values. g 50% inhibitory concentrations [IC50 (µg/mL)] were determined by hemagglutinin inhibitory assay (HAI) against the representative stains from H1N1 and H5N1. Three independent experiments were used to calculate the average values. Values below 50 µg/mL are colored as follows: dark red, extreme reactivity; red, strong reactivity; orange, moderate-high reactivity, yellow, moderate-low reactivity; light green, weak reactivity; green, very weak reactivity; >50, negative reactivity. C12G6, the negative control antibody; FL/2006, control virus. h In vitro microneutralization (IC50) values of C12H5 and the indicated antibodies against the 15 representative strains. Data represent the average values from three independent experiments and are marked with a single symbol. Data are presented as mean values +/– SEM. Group comparisons in h were made by Friedman test ANOVA with Dunn’s multiple comparisons test. *P < 0.05 and ****P < 0.0001 were considered significant. Source data are provided as a Source Data file.

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