Fig. 3: CKI-based analysis of hepatic reprogramming.

a Experimental design of the trans-omics-based analysis of HDFs undergoing hepatic reprogramming by overexpression of FHH. b Heatmap of 15 potentially central PKs predicted by CKI using a threshold of ≥5 of 9 pairwise comparisons; the minus-log transformed p values were calculated for the indicated comparisons. c Expression levels of hepatic functional genes in HDFs overexpressing FHH and individual candidate central PKs for 5 days as quantified by qRT-PCR (n = 3). Overexpression of PIM1 (ALB p < 0.0001, AAT p < 0.0001, ARG p = 0.0020), PIM2 (ALB p = 0.0043, AAT p = 0.0071, ARG p = 0.0133), PIM3 (AAT p = 0.0022, ARG p < 0.0001), TRIB2 (ALB p = 0.0017), TSSK2 (ALB p = 0.0163, AAT p = 0.0027), TSSK3 (ALB p = 0.0077, AAT p = 0.0048), PSKH1 (AAT p = 0.0199), or CAMKV (AAT p = 0.0081) showed significantly changed transcript levels of hepatic functional genes. d Representative image of ALBUMIN immunofluorescence staining in HDFs overexpressing the indicated genes after infection with FHH for 12 days. Scale bars = 100 μm. n = 2 biological replicates. e Expression levels of hepatic functional genes in HDFs overexpressing FHH and shRNAs of candidate central PKs for 5 days as quantified by qRT-PCR (n = 3). Knockdown of PLK1 (ALB p = 0.0011, AAT p = 0.0104, ARG p = 0.0227), PLK2 (ALB p = 0.0038, AAT p = 0.0084, ARG p = 0.0010), PLK4 (ALB p = 0.0001, AAT p = 0.0070), PIM1 (ALB p = 0.0006, AAT p = 0.0113, ARG p = 0.0002), PIM2 (ALB p = 0.0398, AAT p = 0.0010, ARG p = 0.0222), TRIB2 (ALB p = 0.0392), ROCK2 (ALB p = 0.0125, ARG p = 0.0010), or TSSK3 (AAT p = 0.0459, ARG p = 0.0101) showed significantly changed transcript levels of hepatic functional genes. Data are shown as the mean + SD. *p < 0.05, **p < 0.01, ***p < 0.001 (unpaired two-sided Student’s t-test). Source data are provided as a Source Data file.