Fig. 2: Characterization of the ViaA-RavA interaction by Bio-Layer Interferometry (BLI) binding studies. | Nature Communications

Fig. 2: Characterization of the ViaA-RavA interaction by Bio-Layer Interferometry (BLI) binding studies.

From: The AAA+ ATPase RavA and its binding partner ViaA modulate E. coli aminoglycoside sensitivity through interaction with the inner membrane

Fig. 2

a & b BLI measurements of His-ViaA-AviTag (a) or AviTag-ViaA-His (b) coupled on BLI biosensors and RavA, with or without added ADP/ATP. The blue curves correspond to the measured signal while the red curves correspond to the calculated fit using a 1:1 (no nucleotide, ADP) or 2:1 heterogeneous ligand binding (ATP) interaction model. c BLI measurements of AviTAg-ViaA-His coupled on BLI biosensors and either NuoEF (blue curve), NuoEFG (green curve) or FrdA (red curve). SDS-PAGE gels of purified NuoEF, NuoEFG and FrdA are shown on the right. BLI experiments were performed in triplicate, and one representative experiment is shown. Source data are provided as a Source Data file.

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