Fig. 3: Imaging of overexpressed RavA and ViaA by super-resolution microscopy and cellular TEM. | Nature Communications

Fig. 3: Imaging of overexpressed RavA and ViaA by super-resolution microscopy and cellular TEM.

From: The AAA+ ATPase RavA and its binding partner ViaA modulate E. coli aminoglycoside sensitivity through interaction with the inner membrane

Fig. 3

a Single molecule localisation microscopy imaging of E. coli cells overexpressing fluorescently labeled RavA and ViaA by STORM (BC2-RavA, RavA-BC2) and PALM (PAmCherry-ViaA, ViaA-PAmCherry and PAmCherry-CTV). Each experiment was performed in triplicate, and one representative experiment is shown. b Imaging of high pressure frozen, freeze-substituted and sectioned wild-type (WT) E. coli cells and E. coli cells overexpressing His-RavA (RavA-OE) or His-ViaA (ViaA-OE) by TEM. Each experiment was performed in triplicate, and one representative experiment is shown.

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