Fig. 1: Fluorescence of transformed roots and AFLP analysis of nodule pools.
From: Enzymes and cellular interplay required for flux of fixed nitrogen to ureides in bean nodules
a Transgenic hairy roots expressing GFP as selection marker. Images were taken with a binocular equipped with a GFP-long path filter. Scale bar, 500 µm. b AFLP analyses with capillary electrophoresis for mutant identification on three example chromatograms. Green peaks represent JOE-labelled amplicons from XMPP-CRISPR-transformed nodules. Blue peaks represent 6-FAM-labelled (wild type size) amplicons from control nodules transformed with an empty vector. From top to bottom, AFLP analysis of three independent nodule pools showing biallelic null-mutant, homozygous null-mutant and wild type patterns. Analysis was performed once per sample and the mutations of the samples identified as null-mutants were further confirmed by Sanger sequencing.
