Fig. 2: Metabolic alterations in nodules of XMPP, GSDA and XDH genetic variants and fluorescent bodies in xdh nodules.

a Quantification of metabolites of purine catabolism and ureide synthesis in nodule extracts. Error bars are SD, measure of the center is the mean value, n = 5 for control and xmpp, n = 4 for xmpp gsda.2 gsda.3, n = 3 for gsda.2, gsda.3, gsda.2 gsda.3 and xdh. A repeat (n) is a pool of nodules from a single transgenic root. Although different mutant roots / nodules can come from the same plant, in this experiment all null-mutant nodules came from roots of independent plants grown together. Statistical analysis with two-sided Tukey’s pairwise comparison using the sandwich variance estimator. Different letters indicate p values < 0.05. Selected p values are indicated, all p values can be found in the Source Data file. DW dry weight. b From left to right, brightfield image of an xdh nodule cross section with bacteroid infected (dark grey) and uninfected (light grey) cells and precipitates (black dots); confocal fluorescence image of the same cross section with autofluorescent objects (excitation 552 nm, emission 595–622 nm); overlay of both images. Scale bar, 100 µm. Image is representative of at least three nodules from independent transgenic roots. The experiment was performed once in Phaseolus and once in Soybean (Supplementary Fig. 4b, d, e) with similar results.