Table 2 Overview of CRISPR-mediated mutations in target genes of Phaseolus vulgaris

From: Enzymes and cellular interplay required for flux of fixed nitrogen to ureides in bean nodules

 

C

XMPP

GSDA.1

GSDA.2

GSDA.3

XMPP GSDA.2 GSDA.3

GSDA.2 GSDA.3

XDH

NSH1

NSH2

No. of pools

8

15

15

15

15

41

15

23

14

24

Wild typea

8

4

15

6

3

7

4

10

11

21

Chimeric mutanta

0

0

0

0

1

2

1

1

0

0

Incomplete mutanta

0

6

0

6

8

28

7

9

0

0

Null-mutanta

0

5

0

3

3

4

3

3

3

3

  1. aSamples were classified as null-mutant if both alleles of the gene were altered by an insertion or deletion that caused a frame-shift in the gene’s open reading frame. If only one allele was changed in this way, the sample was classified as an incomplete mutant (including cases of biallelic mutations with at least one allele maintaining an intact open reading frame). Only few samples showed a chimeric mutation pattern (more than two peaks in AFLP different from the wild type amplicon). These were omitted from further analysis. C, control (nodules transformed with construct not encoding sgRNAs).
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