Table 3 Specific activities of common bean NSH enzymes alone and in complex

From: Enzymes and cellular interplay required for flux of fixed nitrogen to ureides in bean nodules

Specific activitya, b (µmol min−1 mg−1)

Xanthosine (mM)

0.125

0.125

0.125

Guanosine (mM)

–

0.125

0.250

Only NSH1

1.82 ± 0.06c

0.84 ± 0.11

n.d.

Only NSH2

n.d.

n.d.

n.d.

NSH1/NSH2 complexc

21.77 ± 0.50

17.19 ± 0.55

11.00 ± 2.03

  1. aThe enzymes were Strep- or myc-tagged at the N-terminus.
  2. bErrors are SD (n = 3 technical replicates). All activity values were statistically different with p < 0.05 (see Source Data file).
  3. cThe NSH1/NSH2 complex was purified via Strep-tagged NSH2.
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