Fig. 7: Imprinting fidelity in male KSR/FBS- and FBS + VitC-iPSCs.
From: Imprinting fidelity in mouse iPSCs depends on sex of donor cell and medium formulation
A Heatmap representing the percentage of DNA methylation at the methylated (left) and unmethylated (right) alleles of ICRs for male MEFs as well as male KSR- and FBS-iPSCs, KSR/FBS-iPSCs and FBS + VitC-iPSCs (note: same data as in Fig. 5A for male MEFs, KSR- and FBS-iPSCs). ICRs are shown in rows and samples in columns. Source data are provided as Supplementary Data 2. B Plots display methylated and unmethylated CpGs for each CpG position (in columns) in all the individual reads (in rows) for both maternal and paternal alleles of Dlk1-Dio3 imprinted locus in M FBS+VitC4-6 iPSCs. C Expression analysis of Meg3 gene in subclones of the FBS + VitC5 iPSCs. On top, the scheme represents M FBS+VitC5 iPSCs with a mixed population of cells with normal and abnormal imprinting as estimated by the results in Fig. 7B. The status of Dlk1-Dio3 ICR with normal and abnormal (hypermethylation) imprinting are also displayed (white circle – unmethylated ICR; black circle – methylated ICR; Mat – maternal allele; Pat – paternal allele; orange rectangles – expressed genes; grey rectangles – silenced genes; regions are not drawn to scale). On the bottom, graph represents the RT-qPCR expression analysis of Meg3 expression normalised with the Gapdh housekeeping gene in the subclones of M FBS+VitC5, original M FBS+VitC5 and M FBS1 (normal imprinting) and M FBS5 (hypermethylated phenotype); dashed line represents the Meg3 expression level of the parental M FBS+VitC5 iPSC line; each bar represents data from only one biological replicate. Source data are provided as a Source Data file.
