Fig. 8: Pathway-specific neuron–astrocyte circuitries in the nucleus accumbens.
A NAc spatial map of astrocytic activity above the activation threshold in response to each glutamatergic input. Yellow lines starting from pixel 0 in each subregion were used for quantification (pixel = 50 µm2). B Average CaMPARIRed spatial fluorescence (arb.u.) showing the signal above the activation threshold of the three pathways (mPFC black, 9 slices, 6 mice; Amyg green, 9 slices, 6 mice; vHip blue, 8 slices, 6 mice). Two-way ANOVA, Holm–Sidak test for multiple comparisons; *P < 0.05. For more statistical detail, see Supplementary Table 1. C NAc spatial maps showing overlap colocalization probability between high-density afferent regions and increased astrocytic activity for each glutamatergic input. D Spatial comparisons of the three overlap domains by pixel-to-pixel correlation of different glutamatergic pairs. Pixel value represents the overlap probability (o.p.) for an experimental condition in a specific space (pixel = 50 µm2). Note the low correlation values between conditions, indicating spatial segregation of the overlapping domains. Pearson r correlation, P value (two-tailed). E NAc overlap probability map between high-density afferent regions and increased astrocytic activity for ventral tegmental area (VTA) input. F Comparisons of the % overlap area among VTA and glutamatergic inputs. One-way ANOVA, Holm–Sidak test for multiple comparisons, ***P < 0.001. Error bars express SEM. Source data are provided as a Source Data file.
