Fig. 2: Nascent RNAs colocalize with DNA resection tracts in irradiated HeLa cells.
From: CtIP-dependent nascent RNA expression flanking DNA breaks guides the choice of DNA repair pathway
a Schematic representation of the developed R-SMART technique. b Representative images of DNA resection tracts (black background) and nascent RNAs (white background) upon 5 Gy irradiation, using non-denaturing conditions for BrdU staining and EU labeling, respectively. n = 3 biologically independent experiments. Scale: 10 μm. c Representative quantification of fiber profiles for EU and BrdU intensities from non- and irradiated HeLa cells. n = 3 biologically independent experiments. d Dot graph shows percentage of BrdU and EU signal colocalization on resection tracts generated in non- and 5 Gy-irradiated HeLa and HeLa-RNAseH1 cells. At least n = 60 fields from 3 independent experiments were quantified. P values were calculated using multiple comparison with Ordinary One-Way ANOVA. ***p < 0.0001. Source data are provided as a Source data file.
