Fig. 2: Cell type- and virus-specific reprogramming of lipid metabolism genes.

a–c Effect of ZIKV infection on the expression of rate-limiting enzymes in cholesterol and fatty acid synthesis in a neural progenitor cells (NPCs), b Huh7.5 human hepatoma cells, and c human monocyte-derived macrophages (HMDMs) compared with moDCs. Each symbol represents the baseline expression and the arrows point to the expression in ZIKV+ cells. d SCD expression in tissues that can be infected by ZIKV in order of median expression level (data from GTEx Analysis Release V8). The box shows the median value and extends from the 25th to 75th percentile, while the whiskers extend from min to max values. Points are displayed as outliers if they are above or below 1.5 times the interquartile range. e Unsupervised clustering of normalized expression (Log₂ nTPM) of the indicated genes in the indicated tissues (data from The Human Protein Atlas v21.0)²². f, g qRT-PCR analysis of the relative expression of DHCR7 and SCD in moDCs infected for 24 h with f ZIKV BEH819015 (MOI 1) or g ZIKV PRVABC59 (MOI 1). h RNA-seq analysis of the relative expression of lipid biosynthetic genes (listed in Fig. 1e) in ZIKV+ (SD001) and DENV2+ (UIS353) moDCs vs Mock moDCs at 24 h post-infection. Enzymes catalyzing rate-limiting steps in cholesterol and fatty acid synthesis are highlighted. i qRT-PCR analysis of the relative expression of DHCR7 and SCD in moDCs infected for 24 h with DENV2 (UIS353) at MOI 1. Data are presented as the mean ± SD. Symbols represent moDCs derived from individual donors n = 2 (f) and DHCR7 (i) or n = 3 (g) and SCD (i) biologically independent experiments. *P < 0.05, **P < 0.01 by one-way ANOVA with Tukey’s correction for multiple comparisons (g), (i) and (f) [FACS] or two-sided unpaired t test (f) [None]. Source data and exact P values are provided as a Source Data file.