Fig. 2: Robustness-speed trade-off analysis on generic biomolecular configurations.

a The four possible biomolecular configurations and their simulated performance evaluated in robustness and speed with regulator parameter tuning. The four architectures are converted from the node-based design in Fig. 1a, with the regulator species being either sRNA or protein. In all cases, gene regulations happen at the transcriptional level. Each dot in the robustness-speed plots was computed by analyzing the simulated system dynamics with a 2-hour transcriptional perturbation at equilibrium. b The robustness-speed performance analysis of nine configurations. The x-axis represents the speed metric measured in settling time; the y-axis represents the robustness metric measured in absolute disturbance peak. The plot is generated from simulated dynamics by a 25% randomization on all model parameters. The simulated dynamics are shown in Supplementary Fig.4.