Fig. 6: Mettl3 attenuates protein translation in the brain.
From: Mettl3-dependent m6A modification attenuates the brain stress response in Drosophila
a Control, Mettl3 RNAi, and UAS-Mettl3 upregulation flies were fed 600 uM puromycin for 24 h. (Genotypes: DaGal4 > mCherry RNAi vs DaGal4 > Mettl3 RNAi, ElavGS > UAS-mCherry vs ElavGS > UAS-Mettl3). Brains were dissected from the heads, and protein immunoblots were probed for puromycin incorporation in each sample. Ponceau S staining shows total protein levels. n = 3 biological replicates, 15 brains per replicate. Data presented as mean ± SD, *p < 0.05, **p < 0.01, Student’s two-tailed t-test. p = 0.0085, p = 0.0024. b Control and Mettl3 RNAi flies were HS for 30 min and then fed 600 uM puromycin for 4 h or control and fed puromycin for 4 h. (Genotypes: DaGal4 > mCherry RNAi vs DaGal4 > Mettl3 RNAi). Brains were dissected after 4 h, and protein immunoblots were probed for puromycin levels in each sample. Ponceau S staining shows total protein levels. n = 3 biological replicates, 15 brains per replicate. Data presented as mean ± SD, *p < 0.05, ***p < 0.001, two-way ANOVA with Sidak’s test. p = 0.0125, p = 0.0367, p = 0.0006, p = 0.0476, ns not significant. c ElavGS > mCherry RNAi vs Ythdf RNAi, and ElavGS > UAS-mCherry vs UAS-Ythdf upregulation flies brain puromycin assay. n = 3 biological replicates, 15 brains per replicate. Data presented as mean ± SD, **p < 0.01, Student’s two-tailed t-test. p = 0.0049, p = 0.0003. d ElavGS > mCherry RNAi vs Ythdc1 RNAi, and ElavGS > UAS-mCherry vs UAS-Ythdc1 upregulation flies brain puromycin assay. n = 3 biological replicates, 15 brains per replicate. Data presented as mean ± SD, **p < 0.01, Student’s two-tailed t-test. p = 0.0021, p = 0.0311. Source data and statistical analysis are provided as a Source Data file.
