Fig. 3: FBM MPP2s are functionally distinct from FL and adult BM MPP2s. | Nature Communications

Fig. 3: FBM MPP2s are functionally distinct from FL and adult BM MPP2s.

From: Murine fetal bone marrow does not support functional hematopoietic stem and progenitor cells until birth

Fig. 3

a CFU assay of single MPP2 and LT-HSC cells. b BM and liver MPP2 CFUs frequency. Data are means ± SE of 87–171 single cells from three distinct experiments. c Frequency of CFUs among LT-HSCs from BM and liver. Data are means ± SE of 111–175 single cells from three separate experiments. d MPP2 and LT-HSC transplant. e %CD45.2/GFP PB lineages in BM MPP2 transplant recipients (n = 9–10 recipients/timepoint). f %CD45.2/GFP PB lineages in liver MPP2 recipients (n = 5–9 recipients/timepoint). g %CD45.2/GFP PB lineages in LT-HSC recipients at 16 weeks post-transplant (n = 9–14 recipients/timepoint). Platelets: *P = 0.0102; Erythrocytes: * top, P = 0.0162; * bottom, P = 0.0440. h %Recipients with long-term PB donor reconstitution in (g) (n = 9-14 recipients). §P = 0.1; *p < 0.05. P values determined by Mann–Whitney test, two-tailed. BM bone marrow, CFU colony-forming unit, FBM fetal BM, FL fetal liver, G granulocyte, GEMM granulocyte/erythroid/ monocyte/megakaryocyte, HSC hematopoietic stem cell, LT-HSC long-term HSC, M monocyte, MegE megakaryocyte/erythroid, MPP multipotent progenitor. Source data are provided in the Source Data File. See also Supplementary Fig. 3.

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