Fig. 1: Discovery of tyrosine sulfotransferase from sequence similar network. | Nature Communications

Fig. 1: Discovery of tyrosine sulfotransferase from sequence similar network.

From: Unleashing the potential of noncanonical amino acid biosynthesis to create cells with precision tyrosine sulfation

Fig. 1

a sTyr was biosynthesized from tyrosine and PAPS in the presence of sulfotransferase identified in this study. The resulting biosynthesized sTyr was site-specifically incorporated into thrombin inhibitors, yielding enhanced thrombin inhibition b Sequence similar network (SSN) generated by EFI-EST server with RnSULT1A1 as an input sequence and E value of 5. Each circle stands for a representative node containing sequences with over 80% identity. Edges detection threshold was set at an alignment score of 110. The upper and lower yellow representative nodes are RnSULT1A1 (P17988) and HsSULT1C2 (O00338), respectively. c Schematic representation of reported sulfation reactions of P17988 and O00338. d Screening of tyrosine sulfotransferases with green fluorescent protein assay. All tested proteins are included in the representative nodes of b and NnSULT1C1 is the protein with red label (b). d Data are plotted as the means from n = 2 independent samples. a.u. stands for arbitrary unit.

Back to article page