Fig. 5: Antibody-secreting B cell response to SARS-CoV-2 spike is detected in recipients after the booster dose. | Nature Communications

Fig. 5: Antibody-secreting B cell response to SARS-CoV-2 spike is detected in recipients after the booster dose.

From: A randomized controlled trial of heterologous ChAdOx1 nCoV-19 and recombinant subunit vaccine MVC-COV1901 against COVID-19

Fig. 5

a Representative ELISpot from one ChAdOx1/MVC-COV1901 recipient (S069) and one ChAdOx1/ChAdOx1 recipient (S073). b IgG, IgM, IgA-secreting ASCs frequencies to spike prior to, at day 10 ± 3, and day 28 ± 3 after the booster dose in ChAdOx1/MVC-COV1901 (n = 49) and ChAdOx1/ChAdOx1 (n = 45) groups. The mean frequency of spike-specific ASCs response and its standard deviation at each time point is shown in the figure. Each point represents a single recipient and red line represents the mean. The significance between two groups was determined using a Mann–Whitney test (two-tailed). Day 10 ± 3 IgG, P = 0.0007; Day 10 ± 3 IgM, P = 0.7674; Day 10 ± 3 IgA, P = 0.0010. c The relationship between day 10 ± 3 IgG-secreting ASCs response and serological antibody response at day 10 ± 3 (n = 94) and day 28 ± 3 (n = 94). Antibody titer was measured using a SARS-CoV-2 surrogate virus neutralization test and represented as international units (IU) per mL of serum. The correlation was determined using simple linear regression. d Comparison of day 10 ± 3 ASCs response in short (4-6 weeks, n = 24 in ChAdOx1/MVC-COV1901 group, n = 20 in ChAdOx1/ChAdOx1 group) and long (8–10 weeks, n = 25 in ChAdOx1/MVC-COV1901 group, n = 25 in ChAdOx1/ChAdOx1 group) vaccine dosing intervals. The mean frequency of spike-specific ASCs response and its standard deviation is shown in the figure. Each point represents a single recipient and red line represents the mean. The significance between vaccine dosing intervals was determined using one-way ANOVA with post hoc Dunn’s multiple comparison test. IgG, P = 0.0004, post hoc ChAdOx1/MVC-COV1901 short vs ChAdOx1/MVC-COV1901 long, P = 0.0144; ChAdOx1/MVC-COV1901 short vs ChAdOx1/ChAdOx1 short, P = 0.0004; ChAdOx1/MVC-COV1901 short vs ChAdOx1/ChAdOx1 long, P = 0.0046. IgM, P = 0.4917. IgA, P = 0.0657. *P  <  0.05, **P  <  0.01, ***P  <  0.001, NS not significant, ASC antibody-secreting B cell. Source data are provided as a Source Data file.

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