Fig. 6: T cell response to SARS-CoV-2 spike is present in recipients prior to and after the booster dose. | Nature Communications

Fig. 6: T cell response to SARS-CoV-2 spike is present in recipients prior to and after the booster dose.

From: A randomized controlled trial of heterologous ChAdOx1 nCoV-19 and recombinant subunit vaccine MVC-COV1901 against COVID-19

Fig. 6

a Representative ELISpot from one ChAdOx1/MVC-COV1901 recipient (S062) and one ChAdOx1/ChAdOx1 recipient (S071) against spike (pools 1 and 2), with DMSO as negative control and PHA as positive control. b Total spike-specific T-cell responses (sum of S1 and S2 subunit responses, mean ± standard deviation) prior to, at day 10 ± 3, and day 28 ± 3 after the booster dose in ChAdOx1/MVC-COV1901 (n = 49) and ChAdOx1/ChAdOx1 (n = 45) groups. Each point on violin plot represents a single recipient and black line represents the median. The significance was determined using one-way ANOVA with post hoc Dunn’s test. ChAdOx1/MVC-COV1901, Day 0 vs Day 10 ± 3, P < 0.0001; Day 0 vs Day 28 ± 3, P = 0.0486. ChAdOx1/ChAdOx1, Day 0 vs Day 10 ± 3, P > 0.9999; Day 0 vs Day 28 ± 3, P > 0.9999. The significance between two groups was determined using a Mann–Whitney test (two-tailed). Day 0, P = 0.7022; Day 10 ± 3, P = 0.0039; Day 28 ± 3, P = 0.0053. c Summary data of day 28 ± 3 T cell response in vaccine recipients according to spike peptide pools (mean±standard deviation). The significance was determined using one-way ANOVA with post hoc Dunn’s test. S1 pool comparison, P = 0.0345; S2 pool comparison, P = 0.0334. d The fold change of total spike-specific T-cell responses on day 10 ± 3 (left panel) and 28 ± 3 (right panel) relative to the response prior to the booster dose in short (4–6 weeks, n = 24 in ChAdOx1/MVC-COV1901 group, n = 20 in ChAdOx1/ChAdOx1 group) and long (8–10 weeks, n = 25 in ChAdOx1/MVC-COV1901 group, n = 25 in ChAdOx1/ChAdOx1 group) vaccine dosing intervals (mean±standard deviation). Each point represents a single recipient and red line represents the mean. The significance was determined using one-way ANOVA with post hoc Dunn’s test. Day 28 ± 3, P = 0.0234, post hoc ChAdOx1/MVC-COV1901 long vs ChAdOx1/ChAdOx1 short, P = 0.0273. *P  <  0.05, **P  <  0.01, ****P  <  0.0001, NS not significant, DMSO dimethylsulfoxide, PHA Phytohaemagglutinin, s.f.c. spot-forming cells. Source data are provided as a Source Data file.

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