Fig. 2: PML NBs are required for SUMO2/ubiquitin conjugation in vivo. | Nature Communications

Fig. 2: PML NBs are required for SUMO2/ubiquitin conjugation in vivo.

From: Exploration of nuclear body-enhanced sumoylation reveals that PML represses 2-cell features of embryonic stem cells

Fig. 2: PML NBs are required for SUMO2/ubiquitin conjugation in vivo.

a His-PD of His10-S1 or His10-S2 conjugates from mESCs exposed to arsenic, revealing a rapid ubiquitination of the SUMO2 conjugates only, after their PML-dependent boost. The levels of the various free His10-SUMOs in mESCs are indicated. n = 5 independent experiments. b Representative of His pulldown from His6-HA-SUMO1 mouse liver showing waves of SUMO1 and multi-SUMO1, SUMO2/3 or Ubiquitin (Ub) conjugation with time of arsenic injection. 2 mice per condition are shown over 2 independent experiments with 3 mice per condition.

Back to article page